isoniazid mic and katg gene mutations among mycobacterium tuberculosis isolates in northwest of iran

نویسندگان

seed reza moaddab tuberculosis and lung research centre and paramedical faculty, tabriz university of medical sciences, tabriz, iran

safar farajnia biotechnology research centre, tabriz university of medical sciences, tabriz, iran

infectious and tropical disease research centre, tabriz university of medical sciences, tabriz, iran

immunology research center, tabriz university of medical sciences, tabriz, iran

چکیده

objective(s) isoniazid (inh) is one of the main first line drugs used in treatment of tuberculosis and development of resistance against this compound can result in serious problems in treatment procedures. resistance to inh is mediated mainly by mutation in katg gene that is coded for the catalase enzyme. the proportional method for detection of inh-resistance is time consuming due to the slow growth rate of mycobacterium tuberculosis. in this study, we used pcr-rflp approach for screening of common mutations in katg gene for detection of inh resistance, and compared the results to minimal inhibitory concentration (mic) in m. tuberculosis isolates. materials and methods fifty m. tuberculosis isolates were subjected to study of which, 25 strains were inh-resistant and 25 strains were inh-sensitive. results of 25 inh-resistant strains, the mutation was identified in 56% and 20% in the katg315 and katg463 loci, respectively. in 24% of inh-resistant strains, no mutation was observed in the studied loci. inh mic was <0.2 pg/ml in all sensitive strains whereas among 25 inh -resistant isolates, inh mic was higer than 0.2 pg/ml ranged from 0.2 to 3.2 pg/ml. conclusion our findings revealed that pcr-rflp is capable to identify inh-resistance in more than 76% of inh- resistant m. tuberculosis strains, and could be used for rapid identification of inh resistance. high levels of inh mic were observed in the strains which had mutation in the katg gene in position 315.

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منابع مشابه

Isoniazid MIC and KatG Gene Mutations among Mycobacterium tuberculosis Isolates in Northwest of Iran

Objective(s) Isoniazid (INH) is one of the main first line drugs used in treatment of tuberculosis and development of resistance against this compound can result in serious problems in treatment procedures. Resistance to INH is mediated mainly by mutation in KatG gene that is coded for the catalase enzyme. The proportional method for detection of INH-resistance is time consuming due to the slo...

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Isoniazid MIC and KatG Gene Mutations among Mycobacterium tuberculosis Isolates in Northwest of Iran

OBJECTIVES Isoniazid (INH) is one of the main first line drugs used in treatment of tuberculosis and development of resistance against this compound can result in serious problems in treatment procedures. Resistance to INH is mediated mainly by mutation in KatG gene that is coded for the catalase enzyme. The proportional method for detection of INH-resistance is time consuming due to the slow g...

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Mutations in katG gene sequences in isoniazid-resistant clinical isolates of Mycobacterium tuberculosis are rare.

In this study, a battery of oligonucleotides was directed toward the katG gene and PCR-single-stranded conformation polymorphism (SSCP) analysis was used to search for katG gene deviations in clinical isolates of Mycobacterium tuberculosis from different geographical regions. Since a complete deletion of the katG gene was not found, it is suggested that deletion is not a major mechanism of ison...

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Characterization of Mutations in the Rpob and Katg Gene of Mycobacterium Tuberculosis Isolates From Pasteur Institute of Tehran

Objective: The Rifampicin resistance and susceptibility of Mycobacterium tuberculosis are caused by mutations in the 81-base pair region of the rpoB gene encoding the b-subunit of RNA polymerase. Methods: Isoniazid resistance of M. tuberculosis is related to mutations in inha , oxyR and ahpC genes which 30 to 90 percent of Isoniazid resistance is occurred in 3015 codons of kat...

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Characterization of Mutations in the Rpob and Katg Gene of Mycobacterium Tuberculosis Isolates From Pasteur Institute of Tehran

Objective: The Rifampicin resistance and susceptibility of Mycobacterium tuberculosis are caused by mutations in the 81-base pair region of the rpoB gene encoding the b-subunit of RNA polymerase. Methods: Isoniazid resistance of M. tuberculosis is related to mutations in inha , oxyR and ahpC genes which 30 to 90 percent of Isoniazid resistance is occurred in 3015 codons of kat...

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Multiple mutations in katG and inhA identified in Thai isoniazid-resistant Mycobacterium tuberculosis isolates.

A total of 29 Thai multi-drug-resistant/isoniazid-resistant Mycobacterium tuberculosis isolates were analyzed for mutations in katG from codons 254 to 549, inhA promoter and inhA open reading frame by DNA sequencing and single strand conformation polymorphism. Twenty-five multi-drug resistant isolates exhibited single point mutations (17 isolates at Ser315Thr plus Arg463Leu, 1 at Thr308Pro plus...

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عنوان ژورنال:
iranian journal of basic medical sciences

جلد ۱۴، شماره ۶، صفحات ۵۴۰-۵۴۵

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